Week 3
Nervous Tissue Pre-Lab 3
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Again, no photomicrograph! The cerebral cortex may not stain as intensely. Scan the slide under the 4x objective. If no detail is seen clearly, use the 10x lens. Like the cerebellum it has a cortex on the surface and white matter deeper inside. If under the 10x objective (provided your focussing and light is correct) you see only a featureless mass, you are looking at the white matter. Slowly shift the slide to the other surface. Nucleis of small neurons and glial cells are scattered all over. They do have a layered arrangement, but that is not the issue here. Amongst these, you will recognise at least one (usually two) bands where there are very large cells with triangular bodies and the characteristic cytoplasm and nucleus. In three dimensions these cell bodies are pyramidal in shape. Indeed, they are called pyramidal neurons. In some areas of the cerebral cortex the pyramidal cells are truly large and are called giant pyramidal cells. The dendrites are located at the corners. From the centre of the base a single axon emerges and travels out of the cerebrum. This type of neuron may be difficult to find. This one is optional! |
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